Isolation and Antifungal Activity of Plant Lectins against some Plant Pathogenic Fungi

Mohsen, Soad F. E.; Abbassy, Moustafa A.; Rabea, Entsar I.; Abou-Taleb, Hamdy K.

doi:10.21608/asejaiqjsae.2018.5872

Isolation and Antifungal Activity of Plant Lectins against some Plant Pathogenic Fungi

Document Type : Original Article

Authors

¹ Plant Protection Research Institute, Agricultural Research Center, Sabahia, Alexandria, Egypt

² Department of Plant Protection, Faculty of Agriculture, Damanhour University, Damanhour, Egypt

10.21608/asejaiqjsae.2018.5872

Abstract

Two lectins from seeds of white kidney bean (Phaseoulus vulgaries) andSoybean (Glycine max) were isolated and purified from saline crude extracts of seeds using ammonium sulfate precipitation. The two pure lectins were tested in vitro against two plant pathogenic fungi, Fusarium oxysporumand Rhizoctonia solani. Mycelial growth inhibition technique was performed in accordance with standard protocols to evaluate antifungal activity. Mycelial of both tested fungi were inhibited by the lectins extracts. Results revealed that, the lectin from Phaseoulus vulgaries (LP lectin) had a higher antifungal activity against F. oxysporum (EC₅₀= 5058, 4872 and 3617mg/L, after 48, 72 and 120 h, respectively). Inaddition, the antifungal activity of LP lectin against R. solani was higher than lectin from Glycine max (LG lectin) with EC₅₀ values 6786, 6646 and 5465 mg/L after 48, 72 and 120 h, respectively.

Keywords