Isolation and Identification of Biofilm-Forming Capability Bacteria in Raw Milk Collected from Individual Dairy Farms

Aner, Dina; Mira, Ebaa I.; El-Hawary, Mohamed Y.; Awad, Sameh Ali

doi:10.21608/asejaiqjsae.2025.432799

Isolation and Identification of Biofilm-Forming Capability Bacteria in Raw Milk Collected from Individual Dairy Farms

Document Type : Original Article

Authors

¹ Tanta,EDepartment of Food Science and Technology, Faculty of Agriculture, Tanta University, Tanta 31527, Egypt gypt

² Department of Food Science and Technology, Faculty of Agriculture, Tanta University, Tanta 31527, Egypt

³ Department of dairy science and technology, faculty of Agriculture, ‎Alexandria University, Alexandria, Egypt

10.21608/asejaiqjsae.2025.432799

Abstract

Biofilm formation is recognized as a hazard compromising product safety and raising the possibility of foodborne illness. Thus, it is regarded globally as an emerging public health risk. Various mechanisms contribute to the attachment process and growth of microbial colonies on surfaces in contact with milk during the dynamic process of biofilm formation. This study aims to determine whether bacterial strains are potent biofilm formers by assessing their capacity to form biofilms from raw milk samples collected from cow milk farms in the Egyptian province of Alexandria. A total of 29 samples were chosen randomly from the cow milk samples. Each of the single colonies had morphological characteristics, and their capacity to build biofilm was examined. Various bacterial strains that produce biofilms were identified as Bacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp., Listeria monocytogenes, Pseudomonas spp., Shigella sp., and Enterobacter spp. Also, study the impact of using sodium hydroxide at different concentrations: T1 1%, T2 1.5%, T3 2%, and T4 2.5% for steel and rubber plates. These treatments were applied for 15, 30, 45, and 60 minutes at a temperature range of 70-80°C and used in the Clean-In-Place (CIP) simulation model. The results indicated that there was an increase in the bacterial counts in all treatments compared with the control sample. Also, results showed that a higher bacterial count was noticed in rubber plate treatments compared to steel plate samples. The higher the concentration of sodium hydroxide and the longer the duration, the lower the bacterial growth and total bacterial count.

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