Document Type : Original Article
Authors
1
Genetics department,Faculty of Agriculture (EL-Shatby),Alexandria University,Alexandria,Egypt.
2
Plant Production Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Alexandria 21934, Egypt.
3
Wheat Research Department, Field Crop Research Institute, ARC, Egypt.
Abstract
Drought is considered the most hazardous environmental abiotic stress factors, which adversely affects wheat production (Triticum aestivum L.). Several transcription factor (TF) families, such as WRKY, MYB, and NAC, have an important role in response to drought stress in plants. The investigation of NaC and WRKY genes is considered a new area for research, especially in Egypt. Thus, this study aimed to detect the three genes namely, TaNAC2a, TaWRKY2, and TaWRKY19 by using DNA marker based on encoding regions in different wheat genotypes. In addition to that, to identify the polymorphism in TaNAC2a gene by performing sequence analysis of the central part of exon 2 in seven wheat cultivars. The TaNaC2a, TaWRKY19, and TaWRKY2 genes were detected in most of the tested wheat genotypes. The polymorphic bands detected for TaNAC2a, TaWRKY19, and TaWRKY2 suggest the existence of other alleles at the same locus of these genes. The central part of exon2 was sequenced (260 bp) showing the conserved sequence in all cultivars, except the positions from 813 to 863 were high polymorphic. Detected SNPs related to drought tolerance were not found. The amino acids sequence encoded from the target DNA region was highly conserved from position 282 to 319 in the N-terminal domain in all genotypes except a few positions. Detection of these important genes is considered a critical start point for further study especially gene expression of these genes.
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