Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Hassan, Mona M.; AbdAl-Aziz, Samia A.; Megahed, Asmaa M.; Abdel Rassoul, Hagar A.; Mabrouk, Yasser M.

doi:10.21608/asejaiqjsae.2019.36518

Molecular Identification of Listeria Species from Some Processed Meat Products Using Specific Iap Gene and 16S rRNA Gene

Document Type : Original Article

Authors

¹ Nucleic acids research department, Genetic Engineering and biotechnology Institute, City of Scientific research and Technological Applications, new Borg El Arab, Alexandria, Egypt

² Department of Genetics, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt

10.21608/asejaiqjsae.2019.36518

Abstract

Three kinds of processed meat (Luncheon, Burger , Minced meat) were examined for the presence of Listeria species.Collected samples purchased from local supermarkets were from three different Egyptian companies. All the bacterial isolates were cultured on specific media after pre-enrichment and enrichment cultures. The colonial morphology depends strongly up on the media used and the incubation conditions provided. (Oxford Listeria Agar Base)was used as specific medium for Listeria. The bacterial distribution of Listeria was, 2.22% in Beef burger, 13.6%. in luncheon and there was no bacterial growth in minced meat. In this study; gram staining., morphological characterization, molecular identification using 16S rDNA partial sequencing and specific gene Iap gene of Listeria were carried out.

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