Isoperoxidase Activity in Root- Knot Nematode Sugar Beet Resistance Plants

Document Type : Original Article

Authors

1 Department of Genetics, Faculty of Agriculture, University of Alexandria- (Saba-Bacha).

2 Sugar Crops Research Institute, Department of Genetics and breeding.

3 Sugar Crops Research Institute, Department of. Sugar Crops Pests &diseases Research.

Abstract

The present study was carried out in a pot experiment at Sabahia Agricultural Research Station, Alexandria, Egypt, during successive season 2007-2008 for evaluating activity of peroxidase isozymes (isoperoxidase), in sugar beet plants (susceptible and/or resistant), to root-knot nematode Meloidogyne javanica. Sixteen sugar beet (Beta vulgaris L.), genotypes that displayed differential resistance background to root-knot nematode were employed in this work. Computed damage index classified the sixteen genotypes into three categories four genotypes were found to be susceptible (S), eight moderate resistant (MR), and four genotypes resistant (R). Peroxidase isozyme pattern was studied in control and infected plants.Cluster analysis of (susceptible and resistant), genotypes differentiate control and infected plants in two clusters; which mean nematode infection effect on isoperoxidase activity. The cluster analysis differentiated the eight moderate resistant genotypes in three clusters, cluster number one contained five control and one infected plant, cluster number two contained three control and four infected plants, while cluster number three contained three infected plants. It can be concluded that isoperoxidase in moderate resistant genotypes cannot differentiate between infected and control plants.

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