Improving Barley (Hardeum Vulgare L.) Tolerant to Herbicides Injuries Using Two Methods of Safener Application

A. Houssien, Amel

doi:10.21608/asejaiqjsae.2008.3211

Improving Barley (Hardeum Vulgare L.) Tolerant to Herbicides Injuries Using Two Methods of Safener Application

Document Type : Original Article

Author

Amel A. Houssien

Central of Agriculture pesticides Laboratory Agric. Research center-Sabahia, Alexandria

10.21608/asejaiqjsae.2008.3211

Abstract

In greenhouse, barley seeds (Hardeum Vulgare L) were treated with 1,8 naphthalic anhydride safener using two methods of application. The first method, seeds were coated with naphthalic anhydride (NA) by tow rates (0.5 and 1.0% w/w), and the second were soaking for two hours in potassium salt of naphthalic anhydride (KNA) at concentration 20 and 30 mM in K-phosphate buffer to compare the two methods against the toxic effect of post-emergence application of imazapic (oraban) and fenoxaprop-ethyl ( furour) compared with fenoxaprop-p < /em>-ethyl which have safener in its formulation (puma super) at one field and one and half of field rate. All naphthalic anhydride concentration increase glutathione content, glutathione-s-tansferases activities and total chlorophyll determined 30 days after herbicides application. On the other hand all herbicide rates caused significant decrease in all determined parameters. Naphthalic anhydride succeeded to protect barley seedlings from herbicides injuries and the seedlings still alive even with 1.5 F of field rate. Fenoxaprop-p < /em>-ethyl ( at the ready made formulation) with one field rate gave an increase in all parameter but the rate of increase were less than that with naphthalic anhydride and its safener failed to protect barley seedlings from its high rate.