Effect of Some Mineral Oils Alone and Their Mixtures with Cyhalothrin on The In Vitro Activity of Adenosine Triphosphatase Enzyme (ATPase) Extracted from Spodoptera Littoralis Larvae Brain

Seham M., Ismail; Shaker, Nader

doi:10.21608/asejaiqjsae.2008.3209

Effect of Some Mineral Oils Alone and Their Mixtures with Cyhalothrin on The In Vitro Activity of Adenosine Triphosphatase Enzyme (ATPase) Extracted from Spodoptera Littoralis Larvae Brain

Document Type : Original Article

Authors

Ismail Seham M. ¹
Nader Shaker ²

¹ Central Laboratory of Pesticides, Sabahia, Alexandria, A.R.C.

² Pesticide Chemistry Dept., Fac. of Agrie, Alex., Univ.

10.21608/asejaiqjsae.2008.3209

Abstract

The toxicity effects of three Mineral oils (Folic; Kemesol and National oil) alone and pretreated with Cyhalothrin (Pyrethroid) insecticide on the fourth larval instars of laboratory and field strains of Spodoptera littoralis have been evaluated. The results showed that Folic oil was the most potent against both two strains followed by Kemesol and National oil showed to have less toxic effect. The effect of LC₅₀ of the tested oils and the interaction of Mineral oils with Cyhalothrin on the in vivo inhibition of ATPase from Spodoptera littoralis brain was investigated. Results proved that pretreated of Folic oil with Cyhalothrin incressed the percentage inhibition which found that to be 95.8 and 90.7% for lab and field strains respectively, while the percentage inhibition found by pretreated the Kemesol oil with Cyhalothrin were 90.5 and 88.6% for lab and field strains Spodoptera respectively, in the other hand the percentage inhibition found by pretreated the National oil with Cyhalothrin were 76.2 and 71.3% for lab and field strains Spodoptera respectively. The results emphasized that I₅₀ and K_i values decreased when Folic and Kemesol oil pretreated with Cyhalothrin, so there were significant differences among the chemical combinations, which caused more reduction effect than signal treatment, and they affected enzyme activity by the same trend so results proved that ATPase was sensitive to the Folic and Kemesol oil. The present study open gate that physical toxicant can play important role to increase pesticide toxicity. Generally, Mineral oils pretreated with Cyhalothrin produce a new trend will lead to reduce the field dose of the Pyrethroid insecticides, enhance the role of beneficial insects and reduce the cost of pest control.