Bicontrol of R. Solani AG - 4 with Piperitone Product from Cymbopogon Proximus and Comparison with Bicontrol Agents (Trichoderma Spp)

Document Type : Original Article

Authors

Department of Plant Protection- College of Food Science and Agriculture-King Saud University P.O. Box 2460 Riyadh 11451

Abstract

In vitro antagonism experiments were carried out to study the ability of five biocontrol agents (:Trichoderma harzianum, T. asperellum, T. hamatum, T. virenes and T. viride) and fourteen plant extracts for their effects on the growth of mycelia of R. solani AG-4. T. harzianum was recorded the maximum inhibitor of mycelial  growth followed by  T. asperellum with average of the inhibition  growth 56.82 % and 54.48 %, respectively. Results showed  also that significant antifungal activity was detected for the extracts of  Cymbopogon proximus, and  Ruta chalepensis..  These extracts were effective in inhibiting the mycelial  growth of R. solani AG -4, (at amount/disc, 4 mg) with an  average of 22.59and 21.48 respectively. Comparison studies  of the inhibitory effects of piperitone, biocontrol agents and fungicide (Rizolex) on DSI, length (cm), fresh and dry  weight (gm) of bean plants were also recoded. Treatments  of piperitone in the presence of  R. solani Ag-4 showed the best control of dry root rot and stem bean disease there are  also reduction effect in disease severity with average 1.00,compared with inoculated bean plants with R. solani AG-4  alone with average 3.65. R. solani AG-4. In the presence of T. asperellumor T. harzianum or Fungicide (Rizolex) were also recorded reduction in disease severity with average of  2.41 and 1.66, 1.91, respectively.

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