Physico-Chemical Properties of Chromatin, Proline Content; and Induction of Polyploidy in Stevia rebaudiana (Bertoni)

Document Type : Original Article

Authors

1 Genetics and Breeding Department, Sugar Crops Research Institute, Agricultural Research Center, Egypt.

2 Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University.

Abstract

This work aims at investigating Physico-chemical properties of purified chromatin isolated from Stevia rebaudiana(Bertoni) and estimation of proline content before and after induction of polyploidy, to achieve such purpose, isolation of chromatin was carried out and purified, temperature of  melting, chemical composition of  chromatin,proline content were investigated, polyploidy was proven to be successful. Analysis of data obtained from the physico-chemical properties showed that differential gene expression was detected, given an evidence that the tested treatments were genetically different. However, tow polyploidy plants were observed and it is possible to propagate this martial through tissue culture. This materials provides a good and fretful approach for plant breeder. 

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