Effect of Salinity and Proline Application on The Chemical Content of In Vitro Regenerated Shoots of Mentha Piperita.

Document Type : Original Article

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Department of Floriculture, Ornamental Horticulture and Garden design, Fac. of Agric., Alex. Univ., Alexandria, Egypt.

Abstract

The effect of salt stress and proline treatments on in vitro produced Mentha piperita was investigated in order to find out their effect on p < /strong>roline, charbohydrates and rosmarinic acid accumulation in the shoots tissue of regenerated plants. Apicalportions with axillary buds were obtained from mother plants grown in the Experimental Garden of the Department of Floriculture and Garden Design, Alexandria University and were cultured in MS medium supplemented with 3% sucrose, 8 g/l agar and 0.25 mg/l NAA + 2.5 mg/l BAP in addition to five concentrations of NaCl (0.0, 0.5, 1.0, 1.5 and 2.0 g/l) and proline (0.0, 25, 50, 75 and 100 mg/l). Results showed that increasing NaCl concentration in the medium decreased the total soluble carbohydrates and increased free proline content, whereas, rosmarinic acid (RA) content decreased with the highest NaCl concentrations. 

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