Chlorfenapyr Induce Oxidative Phosphorylation Deficiency in Exposed Rat and the Quinoa Effective Role

Document Type : Original Article

Authors

1 Pesticides Toxicity to Mammals and Aquatic Organisms, Central Agricultural Pesticide Lab., Agricultural Research Center, Alexandria, Egypt

2 Researcher Pesticides Toxicity to Mammals and Aquatic Organisms, Central Agricultural Pesticide Lab., Agricultural Research Center, Alexandria, Egypt

Abstract

The present study investigates uncouple and pro-oxidant effects of chlorfenapyr (CFp) on rat liver and spleen mitochondria respiratory complexes and evaluates the quinoa seeds (Q) (30% from the basal diet) effects on CFp exposed rats. Repeated sublethal oral doses of CFp (180 mg / Kg body weight; 1/3 LD50) was given to rat. Liver and spleen mitochondria were isolated at the end of the fourth week after dosing. Changes in liver and spleen mitochondrial respiration complexes, and concomitant oxidative stress and antioxidant alterations were examined.  Since, CFp provokes a significant reduction in the activity of NADH dehydrogenase (complex I), succinate dehydrogenase (complex II), ubiquinone cytochrome c oxidoreductase (complex III), cytochrome c oxidase (complex IV), superoxide dismutase (SOD), and reduced glutathione (GSH) level. In addition, a significant increase in ATP synthase (complex V) and malondialdehyde (MDA) levels were observed in both liver and spleen mitochondria, indicating clearly an intense peroxidation within mitochondria. So, the results conclude that hepatic and splenic respiratory complexes in rats were severely compromised following CFp exposure. Moreover, Q enhanced diet neutralizes the CFp adverse effect that encourages its use as a mitochondrial enriches source and a protective antioxidant food supplement.
 

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Alexandria Science Exchange Journal
Volume 42, Issue 4 - Serial Number 4
OCTOBER- DECEMBER
October 2021
Pages 809-822

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