Regeneration and Micropropagation Systems of (Stevia rebaudiana) var. sponti

Document Type : Original Article

Authors

1 Laboratory of Biotechnology. Sugar crops Res. Inst., Agric. Res Center.ac. Agric., Saba basha, Alex. Univ.

2 Plant Physiology Department. Sugar crops Res. Inst., Agric. Res. Center

3 Plant Breeding Department , Sugar crops Res. Inst., Agric. Res. Center

Abstract

In order to establish an effective regeneration system of Stevia (Stevia rebaudiana) var. sponti, different concentrations of phytohormones were added to MS basal medium individually or in combination with (IAA, BAP and NAA) as callus induction media. The obtaining results indicated that MS basal medium which containing 0.5 mg/l of NAA and 0.5 BAP was the most efficient and effective medium for callus induction.
For shoot regeneration, different recombination of phytohormones were used, addition of NAA (0.1 mg/l) and BAP (1.0 mg/l) to MS basal medium under light condition caused high increase in shooted calli percentage.
Vigor root system was developed after the addition of 3 mg/l of IBA to MS basal medium, while the addition of high concentration of sucrose (80 g ∕ L) found to be ineffective factor for root regeneration and growth. Micropropagation system was performed by exposure of axillaries and terminal buds to solid MS medium which contained 0.1 IAA and 1.0 BA. Then, clusters were cut into small number of plantlets in MS liquid media without any hormone. This method was found to be more effective than using hormonal medium for micropropagation. 

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