Molecular Detection and Pathogenic Capability of Agrobacterium tumefaciens Isolates from Olive

Jadalla, Nagia M.; Behiry, Said I.; Adam, Mohamed A.M.

doi:10.21608/asejaiqjsae.2018.16883

Molecular Detection and Pathogenic Capability of Agrobacterium tumefaciens Isolates from Olive

Document Type : Original Article

Authors

¹ Plant protection Department, Faculty of Agriculture -Omar Al-mukhtar University. Elbida-Libya

² Agricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt

10.21608/asejaiqjsae.2018.16883

Abstract

Crown gall, caused by Agrobacterium tumefaciens, first reported on olive in Libya. A partial 16SrRNA gene sequence confirmed the identity of the A. tumefaciens bacterial isolates. The Ti-plasmid (induce tumors formation) is the real reason of their pathogenicity. Meanwhile, the prerequisite for the control of A. tumefaciens is to have a rapid and reliable detection method of pathogenic isolates in order to select healthy plants therefore, we detected two plasmidic genes, virD2 gene of the virulence (vir) region (amplified by virD2A/C and virD2A/E primers), and T-DNA (on tms gene). The PCR amplified bands analysis had a correspondence to the pathogenicity assay (carrot tumorgenicity) and the biochemical determents’ of the isolates genus.

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