Laboratory Evaluation of the Toxicity of Silver Nanoparticles Against Housefly, Musca Domestica (Diptera: Muscidae)

Document Type : Original Article

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Department of Applied Entomology, Faculty of Agriculture, Alexandria University, Egypt,

Abstract

The synthesis of nanomaterials is currently one of the most active in nanoscience branches; especially those help improve the human quality life. Silver nanoparticles (AgNPs) are an example of this as it is known to have inhibitory and bactericidal effects and as one of the famous metal nanomaterials in broad Sectors especially in agriculture. It was synthesized by a chemical method and characterized by UV-visible spectroscopy, TEM, and SEM in this present study. The nanoparticles were ranged between 20-50 nm and the shape seem grain with rough ends. The larvicidal activity of silver nanoparticles AgNPs were tested against the third instar larvae of housefly, M. domestica. Six concentrations were used over two different application methods (Dipping and Feeding methods) under laboratory conditions. In general, it was noticed that the larvicidal effects based on LC50 of either method against the housefly.  The LC50 value for AgNPs was 45.5 mg/ml by feeding method in a different concentration (60,40,32,24,16,8 µg/ml). Data showed the moderate effect in larval mortality % and high effect of inhibition of pupation% and inhibition of adult emergence%. Furthermore, the treatment caused some   morphological effects such; abnormalities of larvae and pupae, larval pupal intermediates, deformed adult with crumpled wings. Also, some 

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