Evaluation of Breeding Programs Susceptibility for Two Important Forage Crops Using DNA Barcoding

Aziz.T. Bondok, Abdel

doi:10.21608/asejaiqjsae.2017.1619

Evaluation of Breeding Programs Susceptibility for Two Important Forage Crops Using DNA Barcoding

Document Type : Original Article

Author

Abdel Aziz.T. Bondok

Forage Crops Research Dept. Field Crops Res. Institute, Agriculture Research Center. Giza,Egypt.

10.21608/asejaiqjsae.2017.1619

Abstract

This investigation was carried out to identify and evaluate Medicago sativa andTrifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively.
Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1).
Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.

Keywords