Document Type : Original Article
Authors
1
Floriculture,Ornamental Horticulture and Landscape Gardening, Fac.Agric., Alex.Univ.,Egypt.
2
Floriculture,Ornamental Horticulture and Landscape Gardening, Fac.Agric., Alex.Univ.,Egypt
3
Department of Botanical Garden) Antoniades), Horticultural Research Institute, (A.R.C), Alex, Egypt.
Abstract
This study was carried out in the Tissue Culture Laboratory, Department of Flowers, Ornamental Plants and Landscape Gardens, Faculty of Agriculture (El-Shatby), Alexandria University, Egypt during the period from 2012 to 2015 to establish an efficient and reliable protocol for in vitro propagation and improve of Jatrophacurcas. Seeds of Jatropha were effectiveness of mutation induction by different ways. Planted on Murashige and Skoog media (MS), using different combinations Indol acetic acid (IAA) at 0, 1 and 2 mg/l and benzyl adenine (BA) at 0, 1, 2 and 3 mg/l of kintin (Kin) at 2.0 mg/l and for shoot regeneration .While the media used for rooting was amendmented with different (IAA) at 0, 1 and 2 mg/l and indol butyric acid (IBA) at 0, 1, 2 and 3 mg/l. Oil was extracted from seeds and callus formed on MS medium containing combinations of BA at 0.5, 1 and 1.5 mg/l,(NAA) at 1, 2 and 3 mg/l and 2,4-dichlorophenoxyacetic acide (2,4-D) at 0.5 mg/l.
Regenerated rooted plants were then treated by colchicines or glufosinate ammonium at 1, 2, 3 and 4 mg/l for each. The best medium for shoot regeneration was MS amended with BA at 2 and 3 mg / l and IAA at 1mg/l , while the best rooting medium was MS amended with IAA at 2mg/l and IBA at1 and 2mg/l. For obtaining the highest percentage of oil content from callus, medium at 0.5 mg/l BA combined with 2 mg/l NAA and 0.5 mg/l 2,4-D gave the best results. For the induction of genetic variation glyphosinate ammonium to be added at 3 mg/l, while colchicines had at all concatenation no effect on cell division of Jatropha curcas plant.
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