Impact of Kinetin and Benzyladenine on Growth Performance of Croton in Vitro

Document Type : Original Article

Authors

1 Agriculture research center, Horticulture Institute, Ministry of Agric., Deprt. of Ornamental and Landscape Gardening, Antoniadis, Alex.

2 Plant Production Depit., the Faculty of Agric-saba Basha, Alex.univ

Abstract

This study was carried out in the tissue culture laboratory, Faculty of Agric., Saba Basha, Alex. Univ. to fined reliable protocol for in vitro propagation of Croton (Codiaeum variegatun L.) during the period from 2019 to 2020 was developed. Moreover, nodal explants were used during in vitro culture study for indication of multiple shoots and inoculated on various media with different combinations of NAA as auxin and (BA & KIN) as cytokinin to study compare the effect of the two types of cytokinins on proliferation and development of multiple shoots, and the elongation of the new formed on medium. The best medium for multiplication was a medium supplemented with 2.00mg/l KIN and (0.50 or 1.00) mg/l NAA. Furthermore, the in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 1.00mg/l KIN and NAA each, in turn. The new formed shoot plantlets (rooted plants) were acclimization ex vitro successfully. The survival rate of the ex vitro grown plants was 95%.

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