Peroxidase Isozymes and Proline Content In Relation To Salinity Tolerance in Stevia Plant

Document Type : Original Article

Author

Faculty of Education,Majmaah University, kingdom of Saudi Arabia and Institute of Graduate Studies & Research, Alexandria University,Egypt

Abstract

Stevia (Stevia rebaudiana) plant is an herbaceous perennial used normally as a natural herbal sweetener .Stevia leaves contain several chemicals called glycosides. These glycosides collectively give 100 to 300 times the sweetness of sucrose and are extracted and refined from plants without chemical or enzymatic modifications. The two main glycosides are Stevioside (traditionally 5-10% of the dry weight of leaves) and Rebaudioside A (2-4%). These glycosides are the sweetest compounds. There are also other related compound including RebaudiosideC (1-2%) and Duclosid A and C as well as minor glycosides including flavonoid glycosides; coumarins; cinnamic acids; phenylpropanoids and some essential oils.
This work aims at investigating the role of isozyme variations and proline content in selection and breeding program for salinity tolerance. To achieve such a purpose four landraces of stevia were kindly obtained from the Institute of Sugar Crops and different salinity stresses were conducted.
However, proline acts as a compatible cytoplasmic solute, balancing an accumulation of salts outside of the cytoplasm. Proline levels in salt-shocked leaves, decreased to near control levels within 24 hours of relief of stress.
 The data observed showed that differential gene expression and recommend the use of physio-genetical biomarkers as useful tool to identify the salinity tolerant genome.

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